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Neisser Stain
The Neisser stain is a differential stain in much the same manner that the Gram stain uses more than 1 stain.  The bacteria will either stain Neisser positive (purple) or Neisser negative (yellow).
 
 
Neisser Stain Procedure 
 
Preparation:
 
     Solution 1:
 
          Part A: 0.1 g Methylene Blue, 5 mL 95% Ethanol, 100 mL Distilled Water.
 
          Part B: Crystal Violet (10% w/v in 95% Ethanol) 3.3 mL, 6.7 mL 95% Ethanol, 100 mL Distilled Water
 
     Mix 2 parts of volume of Part A and 1 part volume Part B: Prepare fresh monthly
 
 
     Solution 2:
 
          Bismark Brown (1% w/v aqueous) 33.3 mL
          Distilled Water 66.7 mL
 
Staining Procedure
 
  1. Prepare thin smears on the microscope slides and thoroughly air dry.  Do not heat fix.
  2. Stain 30 seconds with Solution 1;, rinse 10 seconds with water
  3. Stain 1 minute with Solution 2; rinse well with water; blot dry
  4. Examine under oil immersion at 1000x magnification with direct illumination (Brightfield) not phase contrast.  Blue-violet is positive (either entire cell or intracellular granules; yellow-brown is negative

 

 

Video of Neisser Staining Procedure